Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

Katie Shannon

doi:10.1007/978-1-0716-1904-9_6

Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

Katie Shannon

Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Observation of actin at the cortex in dividing cells can be accomplished using the fungal toxin phalloidin conjugated to fluorophores. Protocols for staining both budding yeast and cultured mammalian cells with fluorescent phalloidin are described. This technique can be combined with immunofluorescence to image actin filaments and other proteins involved in cell division simultaneously.

Original language	American English
Title of host publication	Mitosis: Methods and Protocols
DOIs	https://doi.org/10.1007/978-1-0716-1904-9_6
State	Published - Jan 1 2022

Keywords

Actin
Cytokinesis
Fixation
Fluorescence microscopy
Formaldehyde
Phalloidin

Disciplines

Biology

Access to Document

10.1007/978-1-0716-1904-9_6License: Unspecified

https://doi.org/10.1007/978-1-0716-1904-9_6

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title = "Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells",

abstract = " Observation of actin at the cortex in dividing cells can be accomplished using the fungal toxin phalloidin conjugated to fluorophores. Protocols for staining both budding yeast and cultured mammalian cells with fluorescent phalloidin are described. This technique can be combined with immunofluorescence to image actin filaments and other proteins involved in cell division simultaneously.",

keywords = "Actin, Cytokinesis, Fixation, Fluorescence microscopy, Formaldehyde, Phalloidin",

author = "Katie Shannon",

year = "2022",

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doi = "10.1007/978-1-0716-1904-9\_6",

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T1 - Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

AU - Shannon, Katie

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N2 - Observation of actin at the cortex in dividing cells can be accomplished using the fungal toxin phalloidin conjugated to fluorophores. Protocols for staining both budding yeast and cultured mammalian cells with fluorescent phalloidin are described. This technique can be combined with immunofluorescence to image actin filaments and other proteins involved in cell division simultaneously.

AB - Observation of actin at the cortex in dividing cells can be accomplished using the fungal toxin phalloidin conjugated to fluorophores. Protocols for staining both budding yeast and cultured mammalian cells with fluorescent phalloidin are described. This technique can be combined with immunofluorescence to image actin filaments and other proteins involved in cell division simultaneously.

KW - Actin

KW - Cytokinesis

KW - Fixation

KW - Fluorescence microscopy

KW - Formaldehyde

KW - Phalloidin

UR - https://scholarsmine.mst.edu/biosci_facwork/340

UR - https://doi.org/10.1007/978-1-0716-1904-9_6

U2 - 10.1007/978-1-0716-1904-9_6

DO - 10.1007/978-1-0716-1904-9_6

M3 - Chapter

C2 - 34972947

BT - Mitosis: Methods and Protocols

ER -

Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

Abstract

Keywords

Disciplines

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