Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives

Lisa A. Ridnour; Roger A. Winters; Nuran Ercal; Douglas R. Spitz

doi:10.1016/S0076-6879(99)99025-0

Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives

Lisa A. Ridnour
, Roger A. Winters
, Nuran Ercal
, Douglas R. Spitz

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

As glutatione and cellular thiol-related research progresses, the role of these thiols has been extended from acting only in the maintenance of steady-state redox equilibrium, to a proposed involvement in many stressinduced metabolic and bioregulatory functions, including signal transduction and gene expression. 1-3 Glutathione depletion has been identified in various disease processes, including Parkinson's disease and human immunodeficiency virus (HIV) infection. 4,5 Thiols are also known to protect against cytotoxicity associated with exposure to radiation and chemotherapeutic agents. 6'7 Therefore, the evaluation of cellular thiol status may pro-

Original language	American English
Journal	Methods in Enzymology
DOIs	https://doi.org/10.1016/S0076-6879(99)99025-0
State	Published - Jan 1 1999

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Chemistry

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title = "Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives",

abstract = " As glutatione and cellular thiol-related research progresses, the role of these thiols has been extended from acting only in the maintenance of steady-state redox equilibrium, to a proposed involvement in many stressinduced metabolic and bioregulatory functions, including signal transduction and gene expression. 1-3 Glutathione depletion has been identified in various disease processes, including Parkinson's disease and human immunodeficiency virus (HIV) infection. 4,5 Thiols are also known to protect against cytotoxicity associated with exposure to radiation and chemotherapeutic agents. 6'7 Therefore, the evaluation of cellular thiol status may pro-",

author = "Ridnour, \{Lisa A.\} and Winters, \{Roger A.\} and Nuran Ercal and Spitz, \{Douglas R.\}",

year = "1999",

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language = "American English",

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T1 - Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives

AU - Ridnour, Lisa A.

AU - Winters, Roger A.

AU - Ercal, Nuran

AU - Spitz, Douglas R.

PY - 1999/1/1

Y1 - 1999/1/1

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UR - https://scholarsmine.mst.edu/chem_facwork/301

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SN - 1557-7988

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -

Measurement of Glutathione, Glutathione Disulfide, and Other Thiols in Mammalian Cell and Tissue Homogenates Using High-performance Liquid Chromatography Separation of N-(1-pyrenyl)maleimide Derivatives

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