In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures

Qiang Fu; B. Sonny Bal; Roger F. Brown; M. N. Rahaman

doi:10.1016/j.msec.2009.04.016

In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures

Qiang Fu
, B. Sonny Bal
, Roger F. Brown
, M. N. Rahaman

Research output: Contribution to journal › Article › peer-review

Abstract

The objective of the present work was to evaluate the in vitro cellular response to hydroxyapatite (HA) scaffolds with oriented pore architectures. Hydroxyapatite scaffolds with approximately the same porosity (65-70%) but two different oriented microstructures, described as 'columnar' (pore diameter = 90-110 μm) and 'lamellar' (pore width = 20-30 μm), were prepared by unidirectional freezing of suspensions. The response of murine MLO-A5 cells, an osteogenic cell line, to these scaffolds was evaluated using assays of MTT hydrolysis, alkaline phosphatase (ALP) activity, and alizarin red staining. While the cellular response to both groups of scaffolds was better than control wells, the columnar scaffolds with the larger pore width provided the most favorable substrate for cell proliferation and function. These results indicate that HA scaffolds with the columnar microstructure could be used for bone repair applications in vivo.

Original language	American English
Pages (from-to)	2147-2153
Number of pages	7
Journal	Materials Science and Engineering: C.
Volume	29
Issue number	7
DOIs	https://doi.org/10.1016/j.msec.2009.04.016
State	Published - Aug 1 2009

Keywords

Cell culture
Hydroxyapatite

Disciplines

Biology
Materials Science and Engineering

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10.1016/j.msec.2009.04.016License: CC BY

https://doi.org/10.1016/j.msec.2009.04.016

Cite this

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title = "In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures",

abstract = " The objective of the present work was to evaluate the in vitro cellular response to hydroxyapatite (HA) scaffolds with oriented pore architectures. Hydroxyapatite scaffolds with approximately the same porosity (65-70\%) but two different oriented microstructures, described as 'columnar' (pore diameter = 90-110 μm) and 'lamellar' (pore width = 20-30 μm), were prepared by unidirectional freezing of suspensions. The response of murine MLO-A5 cells, an osteogenic cell line, to these scaffolds was evaluated using assays of MTT hydrolysis, alkaline phosphatase (ALP) activity, and alizarin red staining. While the cellular response to both groups of scaffolds was better than control wells, the columnar scaffolds with the larger pore width provided the most favorable substrate for cell proliferation and function. These results indicate that HA scaffolds with the columnar microstructure could be used for bone repair applications in vivo.",

keywords = "Cell culture, Hydroxyapatite",

author = "Qiang Fu and Bal, \{B. Sonny\} and Brown, \{Roger F.\} and Rahaman, \{M. N.\}",

year = "2009",

month = aug,

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doi = "10.1016/j.msec.2009.04.016",

language = "American English",

volume = "29",

pages = "2147--2153",

journal = "Materials Science and Engineering: C.",

number = "7",

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TY - JOUR

T1 - In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures

AU - Fu, Qiang

AU - Bal, B. Sonny

AU - Brown, Roger F.

AU - Rahaman, M. N.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - The objective of the present work was to evaluate the in vitro cellular response to hydroxyapatite (HA) scaffolds with oriented pore architectures. Hydroxyapatite scaffolds with approximately the same porosity (65-70%) but two different oriented microstructures, described as 'columnar' (pore diameter = 90-110 μm) and 'lamellar' (pore width = 20-30 μm), were prepared by unidirectional freezing of suspensions. The response of murine MLO-A5 cells, an osteogenic cell line, to these scaffolds was evaluated using assays of MTT hydrolysis, alkaline phosphatase (ALP) activity, and alizarin red staining. While the cellular response to both groups of scaffolds was better than control wells, the columnar scaffolds with the larger pore width provided the most favorable substrate for cell proliferation and function. These results indicate that HA scaffolds with the columnar microstructure could be used for bone repair applications in vivo.

AB - The objective of the present work was to evaluate the in vitro cellular response to hydroxyapatite (HA) scaffolds with oriented pore architectures. Hydroxyapatite scaffolds with approximately the same porosity (65-70%) but two different oriented microstructures, described as 'columnar' (pore diameter = 90-110 μm) and 'lamellar' (pore width = 20-30 μm), were prepared by unidirectional freezing of suspensions. The response of murine MLO-A5 cells, an osteogenic cell line, to these scaffolds was evaluated using assays of MTT hydrolysis, alkaline phosphatase (ALP) activity, and alizarin red staining. While the cellular response to both groups of scaffolds was better than control wells, the columnar scaffolds with the larger pore width provided the most favorable substrate for cell proliferation and function. These results indicate that HA scaffolds with the columnar microstructure could be used for bone repair applications in vivo.

KW - Cell culture

KW - Hydroxyapatite

UR - https://scholarsmine.mst.edu/biosci_facwork/40

UR - https://doi.org/10.1016/j.msec.2009.04.016

U2 - 10.1016/j.msec.2009.04.016

DO - 10.1016/j.msec.2009.04.016

M3 - Article

VL - 29

SP - 2147

EP - 2153

JO - Materials Science and Engineering: C.

JF - Materials Science and Engineering: C.

IS - 7

ER -

In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures

Abstract

Keywords

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