Abstract
The paper reports on a study that deals with development of a high-performance liquid chromatography (HPLC) method for determination of Indole Acetic Acid (IAA) in Rieger Begonia (Begonia x hiemalis) leaf tissue. The method is based on reverse phase separation on C//1//8 column followed by fluorometric determination. Prior to analysis extensive sample preparation in form of acid-base partitioning and Sephadex column clean-up was found to be essential. The minimum detectable limit of 25 pg was obtained for IAA. The linear range for the method was determined to be 1 multiplied by 10**4. The results obtained by the method compared favorably with more elaborate gas chromatography-mass spectrometry determinations.
| Original language | American English |
|---|---|
| Pages (from-to) | 377-386 |
| Number of pages | 10 |
| Journal | Trace Substances in Environmental Health: Proceedings of University of Missouri's Annual Conferenc |
| State | Published - Jan 1 1984 |
ASJC Scopus Subject Areas
- General Engineering
Disciplines
- Chemistry
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